Cardiac phospholipidome is altered during ischemia and reperfusion in an ex vivo rat model

Acute myocardial infarction (AMI) is the leading cause of death, morbidity, and health costs worldwide. In AMI, a sudden blockage blood flow causes ischemia cell death. Reperfusion after has paradoxical effects may exacerbate injury, process known as ischemic reperfusion injury. this work we evaluated lipidome isolated rat hearts, maintained in controlled perfusion (CT), undergoing global (ISC) or followed by (IR). 153 polar lipid levels were significantly different between conditions. 48 features had q < 0.001 included 8 phosphatidylcholines 4 lysophospholipids, which lower ISC compared to CT, even IR group, suggesting that induces more profound changes than ISC. We observed 16 alkyl acyl phospholipids altered during IR. Overall, these data indicate remodelling possibly damage occurs greater extent reperfusion. The adaptation cardiac described consistent with presence oxidative reflect impact AMI on at cellular level provide new insights into role lipids pathophysiology acute ischemia/reperfusion